In order to assess the knowledge and cleaning practices regarding the disinfection of stethoscopes, the healthcare personnel were administered a questionnaire at the same visit while collecting samples from their stethoscopes. 0D and S0B, respectively) were collected using sterile swabs moistened with sterile saline from one half of the stethoscope to document the baseline microbial load on the diaphragm. The stethoscope was then cleaned by alcohol-based disinfectant (Sterillium), commonly used as hand disinfectant in the hospitals. After cleaning and allowing the stethoscope to dry for 30 s, two samples were further collected using sterile swabs (labelled S1D and S1B) from the other half of the stethoscope’s diaphragm and bell. This division of the stethoscope into two parts was done to obviate the effect of physical removal of microbes by the first swab, thereby affecting the assessment of reduction in microbial load after cleaning, if any. In order to eliminate confusion and maintain uniformity, one of the researchers collected the samples from the stethoscopes whereas another researcher cleaned the stethoscope and administered the questionnaire.
This finding is statistically significant with P < 0
Both the swabs had been labelled towards the date and time from collection in addition to the health-related part of really works of your participant. The participants was indeed tasked another type of identity amount. The fresh gathered examples had been quickly moved towards the microbiology lab and you may have been cultured toward Mineral agar typical and you may incubated for twenty-four h from the 37 °C . Identification of one’s isolates is actually done-by Gram spot and you may practical biochemical tests (Catalase attempt, Coagulase test, Indole try, Methyl red sample, Voges-Proskauer take to, Citrate sample, Oxidase ensure that you Urease take to).
Out of 62 participants, 87% believed that the stethoscope is a potential source of carrying infections from one individual to other. Thirty-three individuals (%) had never cleaned their stethoscopes, 15 (24.2%) cleaned their stethoscopes > 8 weeks previously, two (3.2%) cleaned their stethoscopes 5–8 weeks previously, six (9.6%) cleaned their stethoscopes 1–4 weeks previously and only six (9.6%) individuals had cleaned their stethoscopes < 1 week ago. None of the participants cleaned their stethoscopes after seeing each patient. Among the individuals who cleaned their stethoscopes, only one participant cleaned his/her stethoscope daily. Among the individuals who cleaned their stethoscopes, the majority (86.2%, participants) used alcohol-based disinfectant (Sterillium), followed by a plain cloth (10.3%, 3/29 participants) and soap and water (3.5%, 1/29 participants).
All of the very first swab trials obtained from the latest 62 stethoscopes shown bacterial progress. The fresh samples obtained from 35 stethoscope bells and you may 30 stethoscope diaphragms showed blended gains. To the stethoscope bells, 96 bacteria had been separated and therefore composed of 7 various other genera. Outside of the 96 bacteria isolated out of stethoscope bells, 80 have been Gram-confident cocci (GPC) and you may sixteen was indeed Gram-bad bacilli (GNB). One of the 96 isolates, 25 was indeed probably pathogenic and the people had been surface commensals. On stethoscope diaphragm, a total of ninety-five bacteria was indeed isolated comprising seven some other genera. Outside of the 96 bacteria remote away from stethoscope diaphragm, 79 were GPC and you will thirteen have been GNB. Among the ninety five isolates, twenty-two was in fact probably pathogenic additionally the other countries in the isolates was basically skin commensals. Probably pathogenic isolates were S. aureus, Acinetobacter spp. and you may K. pneumoniae.
Immediately after clean which have a beer-depending disinfectant, K. pneumoniae try separated from just one of one’s stethoscope bells used in an ICU, while on the brand new diaphragm of your own stethoscope included in an ICU, a couple strains regarding K. pneumoniae and one Coagulase-bad staphylococcus (CoNS) were remote and two challenges away from Cons was isolated on the stethoscopes regarding scientific people.
Off 62 stethoscopes, twenty five got possibly pathogenic bacterial increases ahead of clean stethoscopes that have alcoholic beverages-dependent disinfectant. Although not, after washing the stethoscopes, just several got pathogenic development of isolates. 01.